实验教材中“荧光光谱法测定水杨酸”的讨论     

徐彦芹  曹渊  张光辉  陈昌国  鲜晓红 《实验室研究与探索》2011,(7):340-341

为改进教材《荧光光谱法测定水杨酸》,寻找实验所需溶剂CHCl3的替代药品。通过实验,采用1%HAc-CHCl3、1%HAc-C2H5OH、1%HAc-H2O对荧光光谱法测定水杨酸的影响进行比较,并确定1%HAc-C2H5OH为其中最佳溶剂。  相似文献   

HPLC 法测定舒泌通胶囊中绿原酸和蒙花苷的含量     

白文莉  郭文婷  马跃新  杨汝琪  庞仕龙 《昆明师范高等专科学校学报》2015,(3)

建立了 HPLC 法同时测定舒泌通胶囊中绿原酸和蒙花苷的质量分数,采用 SunfireTM-C18柱（4．6 mm ×250 mm,5μm）,柱温为30℃,流动相 A 为甲醇、B 为0．5％冰醋酸,梯度洗脱,流速为1．0 mL／min,检测波长为328 nm．结果表明,二者的线性范围分别为1．0804～216．0804μg／mL 和1．0804～81．7200μg／mL．平均回收率分别为101．0％和9．3％,RSD 分别为3．4％和3．3％（n ＝9）．该方法准确、可靠,具有良好的重复性和稳定性,可用于舒泌通胶囊中绿原酸和蒙苷花的质量分数测定．  相似文献   

运动对RBP4诱导的脂代谢异常鼠肝脏ACC1 表达和脂肪合成的影响          下载免费PDF全文

张明军 《河北体育学院学报》2017,31(1):54-59

目的:研究运动对RBP4诱导的脂代谢异常鼠肝脏ACC1表达和脂肪合成的影响.方法:重组人RBP4注射建立脂代谢异常鼠模型.设一次性运动组(RBP4 One-time exercise group,ROE 组)、8周有氧运动组(RBP4 aerobic exercise group,RAE组)和安静对照组(RBP4 control group,RC组).结果:ROE组血清RBP4水平与RC组相比显著降低(P＜0.05),血清TG和肝脏TG含量无显著改变(P＞0.05).RAE组血清RBP4、TG和肝脏TG含量与RC组和ROE组相比均显著降低(P＜0.01).与RC组相比,ROE组和RAE组肝脏ACC1mRNA表达均显著降低(P＜0.01).与RC组相比,ROE组ACC1蛋白表达无显著改变(P＞0.05).与RC组和ROE组相比,RAE组ACC1蛋白表达均显著降低(P＜0.01).结论:高RBP4显著增加小鼠肝脏ACC1基因和蛋白表达增加,促进肝脏脂肪合成.8周有氧运动抑制了RBP4在肝脏的脂肪合成促进作用,改善脂代谢,机制涉及降低RBP4水平、减少肝脏ACC1基因和蛋白表达以及TG的合成.  相似文献   

低氧运动调控PPARα表达的研究述评          下载免费PDF全文

鞠丽丽 《河北体育学院学报》2016,30(6):77-81

过氧化物酶体增殖物激活受体α(PPARα)是参与调控脂代谢、抑制炎症反应和促进脂肪细胞分化的重要细胞核受体.而低氧运动可通过激活一系列分子应答机制,促进机体不同组织PPARα表达及其介导的信号分子通路重新整合,从而改变机体脂代谢体系.利用文献资料法,梳理低氧刺激对机体PPARα表达影响的相关研究,总结不同组织PPARα对其低氧运动产生代偿性适应的可能分子机制,旨在更好地解释低氧环境下运动机体PPARα在调控脂代谢中的作用.  相似文献   

Chlorogenic acid prevents acetaminophen-induced liver injury: the involvement of CYP450 metabolic enzymes and some antioxidant signals     

Chun Pang  Yu-chen Sheng  Ping Jiang  Hai Wei  Li-li Ji 《Journal of Zhejiang University. Science. B》2015,16(7):602-610

Chlorogenic acid (CGA), a polyphenolic compound, is abundant in fruits, dietary vegetables, and some medicinal herbs. This study investigated the prevention of CGA against acetaminophen (AP)-induced hepatotoxicity and its engaged mechanisms. CGA reversed the decreased cell viability induced by AP in L-02 cells in vitro. In addition, CGA reduced the AP-induced increased serum levels of alanine/aspartate aminotransferase (ALT/AST) in vivo. The effect of CGA on cytochrome P450 (CYP) enzymatic (CYP2E1, CYP1A2, and CYP3A4) activities showed that CGA caused very little inhibition on CYP2E1 and CYP1A2 enzymatic activities, but not CYP3A4. The measurement of liver malondialdehyde (MDA), reactive oxygen species (ROS), and glutathione (GSH) levels showed that CGA prevented AP-induced liver oxidative stress injury. Further, CGA increased the AP-induced decreased mRNA expression of peroxiredoxin (Prx) 1, 2, 3, 5, 6, epoxide hydrolase (Ephx) 2, and polymerase (RNA) II (DNA directed) polypeptide K (Polr2k), and nuclear factor erythroid-2-related factor 2 (Nrf2). In summary, CGA ameliorates the AP-induced liver injury probably by slightly inhibiting CYP2E1 and CYP1A2 enzymatic properties. In addition, cellular important antioxidant signals such as Prx1, 2, 3, 5, 6, Ephx2, Polr2k, and Nrf2 also contributed to the protection of CGA against AP-induced oxidative stress injury.  相似文献   

阿魏酸镧配合物的合成与表征及热化学研究     

黄熠  唐伟军  蔡罗蓉  张芳  张伟 《湘南学院学报》2015,(2)

用阿魏酸与镧的氯化物合成了一种新的稀土配合物,采用红外光谱等对配合物进行了表征,确定了化学式为La(C10H9O4)3·2H2O,在常压、298.15K下,分别测定了阿魏酸、氢氧化钠、七水氯化镧、阿魏酸镧、氯化钠和水在混合溶剂(丙酮：无水乙醇：4mol·L-1HCl ＝1：1：1)中的溶解焓,并根据热化学原理得出了298.15 K下配合物的标准摩尔生成焓为(-2856.59±2.52)kJ·mol-1.  相似文献   

The effect of l-citrulline and watermelon juice supplementation on anaerobic and aerobic exercise performance     

Paul T. Cutrufello  Stephen J. Gadomski  Gerald S. Zavorsky 《Journal of sports sciences》2015,33(14):1459-1466

Citrulline has been proposed as an ergogenic aid, leading to an interest in watermelon given its high citrulline concentration. The aim of this study was to determine the effects of a single, pre-exercise dose of l-citrulline, watermelon juice, or a placebo on the total maximum number of repetitions completed over 5 sets, time to exhaustion, maximal oxygen consumption (VO₂max), anaerobic threshold, and flow-mediated vasodilation. A randomised double-blind within-participants study design was used to examine these effects among 22 participants (n = 11 males). Supplementation included either a 7.5% sucrose drink containing 6 g of l-citrulline, 710 mL of watermelon juice (~1.0 g citrulline), or a 7.5% sucrose placebo drink. Supplementation was administered 1 or 2 h before exercise testing to investigate a timing effect. There was no significant effect between the three supplements for the total number of repetitions, time to exhaustion, VO₂max, anaerobic threshold, or flow-mediated vasodilation. There was also no interaction observed relative to gender or supplement timing (P > 0.05). A single dose of l-citrulline or watermelon juice as a pre-exercise supplement appears to be ineffective in improving exercise performance; however, greater doses of l-citrulline have been shown to be safe and are currently left unexamined.  相似文献   

规律运动对成年人糖脂代谢影响的Meta分析          下载免费PDF全文

孙小刚 《河北体育学院学报》2018,32(1):87-96

目的：评价规律运动对成年人糖脂代谢的影响效果,为预防和控制糖脂代谢紊乱提供科学的锻炼指导。方法：检索中国知网、万方、维普数据库,收集关于规律运动对成年人糖脂代谢影响的研究成果,采用Cochrane偏倚风险评估工具对文献质量进行评估,采用RevMan 5.2进行Meta分析。结果：共纳入17篇研究文献,其中较高质量的2篇、中等质量的8篇、低等质量的7篇;Meta分析结果显示,与对照组相比较,规律运动能够有效降低机体的血糖、甘油三酯和总胆固醇含量,增加高密度脂蛋白含量,但规律运动对于降低低密度脂蛋白含量没有显著效果;进一步亚组分析结果显示,规律运动的干预时间无论长短均与血糖含量存在显著差异,但多于3个月时才能降低甘油三酯、总胆固醇含量,增加高密度脂蛋白含量。结论：规律运动能够有效改善糖脂代谢水平,当运动持续时间多于3个月,改善糖脂代谢水平的效果将更明显。  相似文献   

HPLC法同时测定热淋清胶囊中没食子酸、原儿茶酸、槲皮苷和槲皮素的含量     

杨芳芳  余伯阳 《凯里学院学报》2011,29(3):84-86

建立热淋清胶囊中没食子酸、原儿茶酸、槲皮苷和槲皮素的HPLC定量方法.采用Wa-ters色谱柱,乙腈-0.4%磷酸为流动相梯度洗脱,流速1.0 mL/min,检测波长254 nm,柱温40℃.没食子酸、原儿茶酸、槲皮苷和槲皮素的线性范围分别为1.376~9.632μg,0.041 6~0.291 2μg,0.061 5~0.430 5μg,0.006 702~0.046 914μg;平均回收率分别为98.71%(RSD=1.17%)、98.77%(RSD=1.92%)、99.19%(RSD=1.92%)、100.39%(RSD=1.99%).所建立的方法准确、稳定,重现性好,可用于热淋清胶囊中有效成分的定量分析.  相似文献   

Diagnosis of Major Organic Acidurias in Children: Two Years Experience at a Tertiary Care Centre     

M. P. Narayanan  Vaidyanathan Kannan  K. P. Vinayan  D. M. Vasudevan 《Indian journal of clinical biochemistry : IJCB》2011,26(4):347-353

Organic acid disorders are inherited metabolic disorders in which organic acids accumulate in tissues and biological fluids of affected individuals. Classical organic acidurias include methylmalonic aciduria, propionic aciduria, isovaleric aciduria and maple syrup urine disease (MSUD). They are considered the most frequent metabolic disorders among severely ill children. Patients frequently present with acute symptoms early in life. 420 cases clinically suspected to have organic aciduria, with upper age limit of 12 years for a 2-year period (January 2007–December 2008) were enrolled into this study. Metabolic acidosis and neurological symptoms were the most common signs. Screening tests and thin layer chromatography were done for detection of organic acidurias. Identification and quantitation of organic acids in urine and quantification of amino acids in blood were done by high performance liquid chromatography. Out of 420 patients, 45 patients (10.7%) were found to have organic acidurias. 15 cases of methylmalonic aciduria, 16 cases of propionic aciduria, 13 cases of MSUD, and one case of isovaleric aciduria were diagnosed. Results demonstrate the importance of testing for organic acidurias. Since organic aciduria may cause irreversible brain damage if not treated, we recommend selective screening amongst severely ill children despite implied extra costs.  相似文献